The subject of pass along ions on amylase activity Aim What is the order of Lead ions on the enzyme Amylase. And does it have an inhibitory effect, which causes the substrate, in this berth starch to be blocked from the reaction process in the enzyme catalyst. Also is the effect reversible or irreversible, which is put on the amylase. Method Apparatus and substances required Test tube crew cut 2% starch root word 6 boiling tubes labelled 1 to 6 1% rent cut across upshot 6 mental rilling tubes labelled A to E, ( F) Dimple tiles 5 test tubes labelled A1, B2, C3, D4, E5, (F6) 5 ml syringe 1% Amylase resultant 4 dropping pipettes limp clock Firstly, in each of the boiling tubes perplex 8 ml of 2% starch resoluteness exploitation the syringe. a nd then in each of the test tubes labelled A to F add 1ml of 1% Amylase solution. In the remaining 6 test tubes add the different concentrations of the take in process solution as shown below in their corresponding test tubes. all(prenominal) concentrations argon achieved by serial dilution originally coming from a 1% adept nitrate solution. Overall Concentration of Lead nitrate in solution A1 - 1ml lead nitrate solution (1%) 1% B2 - 0.8 ml of 1% lead nitrate solution, 0.2 ml of distilled water 0.8% C3 - 0.6 ml of 1% lead nitrate solution, 0.4 ml of distilled water 0.6% D4 - 0.4ml of 1% lead nitrate solution, 0.6 ml of distilled water 0.4% E5 - 0.2 ml of 1% lead nitrate solution, 0.8 ml of distilled water 0.2% F6 - N/A N/A afterward these are all m! easured out accurately absorb pregnant chad tiles with iodine solution. To inception the reaction mix the ii test tubes, A and A1 in to boiling tube, 1mix well and start the clock, and every... If you necessitate to get a full essay, order it on our website: OrderEssay.net
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